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Example of a PAT-ChIP experiment performed in our Lab

(Human - Breast Cancer FFPE-sample) 

PAT-ChIP-Seq: chromatin was isolated from FFPE sections (10um each) of Breast Cancer (FFPE archive sample) and immunoprecipitated using anti-H3K4me3 antibody. The immunoselected DNA was sequenced by NGS technology. We report, as example, the screenshots of three different loci (highlighted in red) such as Gapdh, Vcl and Hapln1.

MOLECULAR PATHOLOGY LAB. “PaoLa”

 

University of Urbino

Dept. of Biomolecular Sciences             

via Arco d'Augusto, 2 

61032 - FANO (PU) - Italy

Staff Members:

Prof. Mirco Fanelli                  - Head of Laboratory         email               Tel: +39.0722.304951

Dr.    Stefano Amatori           - Senior Scientist                email               Tel: +39.0722.304956

Dr.    Francesco Cantatore    - B.S. - PhD Fellow             email               Tel: +39.0722.304965

Dr.ssa Martina Rusin              - B.S. - EIO Fellow              email               Tel: +39.0722.304965

Dr.ssa Roberta Borgognoni   (Secretary's office)           email                Tel: +39.0722.304952

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