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Example of a PAT-ChIP Seq experiment performed in our Lab

(Human - Normal Colon FFPE-sample)  

PAT-ChIP-Seq: chromatin was isolated from FFPE sections (10um each) of normal human Colon (FFPE archive sample) and immunoprecipitated using anti-H3K4me3 antibody. The immunoselected DNA was sequenced by NGS technology. We report, as example, the screenshots of three different loci (highlighted in red) such as ACTINB, YY1 and SIN3A (the corresponding CpG islands are showed in green below each enriched region). The screenshots report also other enriched regions and the expected correlation with the CpG island (unpublished results).

MOLECULAR PATHOLOGY LAB. “PaoLa”

 

University of Urbino

Dept. of Biomolecular Sciences             

via Arco d'Augusto, 2 

61032 - FANO (PU) - Italy

Staff Members:

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Prof.    Mirco Fanelli                  Head of Laboratory                       phone: +39.0722.304951

Prof.    Stefano Amatori             Senior Scientist                            phone: +39.0722.304956

Dr.ssa Enrica Sordini                  B.S. - PhD Fellow                          phone: +39.0722.304965

Dr.ssa Chiara Giolitto                B.S. - PhD Fellow                           phone: +39.0722.304965

Dr.       Luca Visotti                     B.S. - Research Fellow                   phone: +39.0722.304965

Dr.ssa Roberta Borgognoni     (Secretary's office)                         phone: +39.0722.304952

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